Sunday, February 01, 2015

Blood Test to Determine Vitamin/Mineral/Nutrient Levels - #BodyBio

My naturopathic doctor is great!  I'm passing on what she has recommended to me to whomever it can help. 
If you have a vitamin/mineral deficiency, you could go years not feeling well and struggling.  Your regular PCP/MD just don't do a thorough enough test to determine all your needs.  Doctors are on the vitamin D train now... hopefully, but what else might your body be lacking? 
BodyBio is a blood test that will determine vitamin levels/needs.  Check it out if you think you are deficient OR if you have a disease like Crohn's colitis.  Inflammation in the digestive system will hinder the absorption of the nutrients from your foods and supplements.  Most people with a digestive disease probably have malabsorption issues and nutrient deficiencies. 
Just from the top of my head, B vitamins, iron, D, mag, calcium, zinc, C, iodine, K 

Here's the link to the main page for the test - BioCell|Home:

This is a screenshot of one of the example diagnostic pages .  

Saturday, January 31, 2015

HSP70 Could Fix Gene Mutations of NOD2 in People w/ Crohn's Disease

I have all these drafts that I never published at the time that I am going to post now.  My focus and concentration is not on the management of symptoms to quiet Crohn's anymore.  There's too much scientific data that suggests that Crohn's is caused by a bacteria in people that are immune deficient (can't fight off the pathogen and kill it the way normal immune systems do - that's why some people get Crohn's and others remain healthy).
My goal is to be well and have health again. I do understand that everyones approach to handling their disease is unique to the individual, so if I can provide anything that can help them, I will do that.
Happy Saturday.. Yay, it's sunny!

Researchers have uncovered a protein that stabilizes Crohn's disease gene
Researchers say they have identified a protein that stabilizes another protein involved in Crohn's disease. University of Delaware researchers discovered how certain proteins can prevent gut bacteria can trigger an abnormal immune response to lead to inflammation associated with Crohn's and other inflammatory bowel disorders.
New target for treating Crohn's disease
Past research has focused on the role of gut bacteria as a contributor to Crohn's disease. The new research conducted by Catherine Leimkuhler Grimes, assistant professor of chemistry and biochemistry at UD, and Vishnu Mohanan, doctoral student in biological sciences focused on mutation of a gene called NOD2 — nucleotide-binding oligomerization domain containing protein 2 - that is strongly associated with Crohn's disease.
Mohanan discovered HSP70 that stands for "heat shock protein 70" plays a role in helping the body attack "bad" gut bacteria, which essentially "fixes" mutations of NOD2.
HSP70 is referenced as a chaperone molecule that helps proteins maintain their three dimensional shape.
According to a press release, "..we stumbled on this chaperone molecule," says Mohanan, who was the lead author of article.published in the July 4, 2014 Journal of Biological Chemistry.''

Sunday, December 07, 2014

Infographic Snapshot of the Lifecycle of #MAP in People w/ #Crohns

Great infographic image of the life cycle of MAP.  

The part of this picture that should jump out at you is the statistic on the bottom left. citation # 3

92% !!!....... this is madness!  This isn't new news people.  So why doesn't anyone know about MAP and it's relation to Crohn's?  I'm not talking about lay persons, but people in the profession of helping people get better who are living with this disease.  They don't acknowledge it.  The medical community flat out ignores the science and the 30 years or more of research that exists.   People need to take charge of their life and health and start fighting to know something.  Ask questions and demand an answer is where I'd start.  

Click link below to view image larger from original source:

Saturday, September 20, 2014

Crohn's - MAP Bacteria Connection *Video*

Informative 3 min. YouTube video to raise awareness about the Crohn's-MAP bacteria connection.  How humans are exposed and vulnerable to MAP - There are now ways to test for MAP.   
I also posted the links that were provided on the YouTube page under the video for further reading/info.

- The Crohn’s MAP Vaccine
A modern therapeutic Vaccine against MAP has been created. Preliminary studies in animals have shown it to be safe and effective. Next, a trial in humans is needed to take the vaccine from lab to clinic.
Professor John Hermon-Taylor, King's College London

Crohn's Disease Info Center:

Providing the highest quality medical care, Shafran Gastroenterology Center, IBD Center-of-Excellence in Florida:

CDD The Centre for Digestive Diseases, Crohn's Disease:

Efficacy and Safety of Anti-MAP Therapy in Adult Crohn's Disease (MAPUS), RHB-104:

RHB-104 is a proprietary antibiotics drug combination for treatment of Crohn’s disease:

Sunday, August 17, 2014

Individual Inflammatory Bowel Disease Therapies from Biopsied Epithelial Cells

That would be cool.  Getting treated based on needs that addresses the missing  component. I'll be positive and not bitch about the fact that they aren't looking to zoom up on what the cause is that is changing the cells in the first place.  Something is causing them to respond to certain factors a certain way.  That's a post for another day.  This study was positive though.  Maybe this can lead to treatments that can improve the quality of life in people that suffer from IBD/gastrointestinal disturbances. We can only hope that treatments will be better and safer.  So, Washington University researchers used biopsy cells, taken from patients during a routine endoscopy to study if epithelial cells could be grown in the lab if therapeutic conditions were provided.  According to the article, cells were obtained and placed in "a high concentration of conditioned media containing the factors critical for growth (Wnt3a, R-spondin and Noggin)". Within 2 weeks, the result from these conditions were positive.  They showed that epithelial cells formed "functional, polarised monolayers covered by a secreted mucus layer when grown on Transwell membranes". They also exposed the cells to bacteria (e. coli) to see how they would respond when exposed to harmful bacteria changes.  The results were favorable.   

Intestinal Epithelial Cells
Technology for the growth of human intestinal epithelial cells could help scientists develop individualized therapies for inflammatory bowel disease and other gastrointestinal conditions. This is the conclusion of a group of researchers at Washington University School of Medicine in St. Louis, who recently published a study, entitled, “Development of an enhanced human gastrointestinal epithelial culture system to facilitate patient-based assays,” in the Journal Gut.
For this to be possible, the team explained in the abstract of their study that epithelial cells must be enhanced so that biopsies from patients can be used to generate cell lines (which are populations of cells in culture with the same genetic makeup) in a short time frame, so that the analysis can be made accurately.
In two weeks, researchers were able to create 65 human gastrointestinal epithelial cell lines from biopsies of 47 patients, taken during routine endoscopic screening procedures. They used a high concentration of conditioned media containing the factors critical for growth, which resulted in a rapid expansion of the proliferative stem and progenitor cells. In addition, the team used a combination of lower conditioned media concentration and Notch inhibition to differentiate these cells for further research.
Results showed that under differentiation conditions, intestinal epithelial spheroids developed mature epithelial lineages in specific regions. These cells formed functional, polarized monolayers covered by a secreted mucus layer when grown on Transwell membranes.
After this process, researchers conducted experiments to determine how the cells interacted with bacterial pathogens like Escherichia coli. They found the cells also demonstrated novel adherence phenotypes with various strains of pathogenic Escherichia coli.
Ultimately, these findings can help testing of new drug targets, as well as developing vaccines. Furthermore, they will play a crucial role in understanding how human cells interact with beneficial and harmful microbes, researchers believe.

Link to the abstract journal article in Gut - Gut doi:10.1136/gutjnl-2013-306651